IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/04/Shan Shen
Prepare the samples of the bi-stable cells:
Prepare the first sample, the residual was diluted for a fold.
Prepare one sample with the same method each hour.
Check those samples using the flow cytometer. There aren’t strong red and green controls, so that the results are not convincing.
Check the parts:
|Number||Size of backbone||Size of the insert||Location in the plate||Description||Plasmids|
|BBa_C0040||1-4A||Tet repressor + LVA|
|BBa_C0012||1-2O||lacI + LVA|
|BBa_C0080||1-14L||araC + LVA|
|BBa-J3033||2-4O||B0034 + LuxR|
|BBa_R0010||1-1D||LacI regulated promoter|
Transformation for the parts above.
The incubation was started.
Pick the pZSA GREEN and RED for incubation, 3-13M as a red control, 2-9B as a green control, 1-1D as a negative control.