IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/04/Shan Shen

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Prepare the samples of the bi-stable cells:

9:30
Prepare the first sample, the residual was diluted for a fold.
Prepare one sample with the same method each hour.

17:30
Check those samples using the flow cytometer. There aren’t strong red and green controls, so that the results are not convincing.

19:30
Check the parts:

Number Size of backbone Size of the insert Location in the plate Description Plasmids
BBa_C0040 1-4A Tet repressor + LVA
BBa_C0012 1-2O lacI + LVA
BBa_J09250 2-9B Constitutive GFP
BBa_C0080 1-14L araC + LVA
BBa_K093012 3-13M Constitutive GFP
BBa-J3033 2-4O B0034 + LuxR
BBa_R0010 1-1D LacI regulated promoter
BBa_B0025 1-2E Double terminators
BBa_I14033 1-18P

22:30
Transformation for the parts above.

1:45
The incubation was started.

2:00
Pick the pZSA GREEN and RED for incubation, 3-13M as a red control, 2-9B as a green control, 1-1D as a negative control.