IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/12/Zhangs

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Determine the concentration of 1-5J plasmid by spectrophotometer.
1-5J② 7.55ng/μL×50=377.5ng/μL
1-5J③ 2.53ng/μL×50=126.5ng/μL

Double digestion for 1-5J+CI+1-23L insert again

1.5μL XbaI
1.5μL PstI
2μL 10×M buffer
3μL/7μL 1-5J② plasmid/1-5J③ plasmid
12μL/8μL ddH2O
20μL Total