IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/11/Zhangs

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Store the strain of 1J①、11N②、2I①、1H④、2M①、5N①、2G① and send to sequence
7 samples in total to be sequenced:
T7P+1J+CI+T①;
T7P+5N+CI+T①;
T7P+2G+CI+T①;
T7P+2I+CI+T①;
T7P+2M+CI+T①;
T7P+11N+CI+T②;
T7P+1H+CI+T④.
The results demonstrates that
T7P+1J+CI+T①;
T7P+2I+CI+T①;
T7P+1H+CI+T④;
T7P+2M+CI+T①;
T7P+2G+CI+T①;
ARE THE TARGET PRODUCTS. While T7P+5N+CI+T①; T7P+11N+CI+T② failed.

Miniprep for 5J+CI①~⑤ and T7p+2K①~⑤
Electrophoresis to identify
Result 1 (from left to right)
T7p+1-2K+CI+T①-⑤(plasmid), T7p Plasmid Control, Marker
PKU 20090711 Zhangs 1.JPG
The T7p+1-2K+CI+T①-⑤ are the target plasmids.
Result 2 (from left to right)
1-5J+CI+T①-⑤(plasmids), RBS Plasmid Control, Marker
PKU 20090711 Zhangs 2.JPG
Double digestion for 1-5J② insert:

1.5μL XbaI
1.5μL PstI
2μL 10×M buffer(failed)
6μL 1-5J② plasmid
9μL ddH2O
20μL Total