IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/09/Zhangs

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Transformation of 2 samples: T7P+1-2K+CI+T & 1-5J+CI+1-23L.
PCR identification of T7P + [1-2G, 1-11N,1-1H, 1-5N, 1-2I, 1-2M, 1-1J] + CI + T, 5 samples for each RBS samples, 35 samples in total.
System for PCR identification:

5μL Master mix
0.25μL Primer For
0.25μL Reverse
4.5μL ddH2O
0μL template
10μL Total