IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/23/Zhangs

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Double digestion for Terminator vector
Digestion system:

30μL Terminator plasmid
5μL 10×M beffer
1μL EcoRI
1μL XbaI
13μL ddH2O
50μL Total

After 90min digestion, add: 1~2μL CIAP+5μL CIAP buffer

Ligate the CI insert and Terminator vector.

Transforming the CI insert + Terminator vector ligation product.