IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/16/Min Lin
From OpenWetWare
Jump to navigationJump to search
MiniPrep 1-18P and 2-4O plasmid
Enzyme Digestion of 1-18P and 2-4O plasmid
| SpeI | 1ul |
| PstI | 1ul |
| 10xH buffer | 2ul |
| 1-18P Plasmid | 2ul |
| ddH2O | 13ul |
| XbaI | 1ul |
| PstI | 1ul |
| 10xM buffer | 2ul |
| 2-4O Plasmid | 10ul |
| ddH2O | 6ul |
Several Trials and failures, 2-4O can not be digested normally, at least be TaKaRa Enzymes.
Check up the parts and found
BBa_J09855 Constitutive LuxR with pLuxR 1-9H pSB1A2
