IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/09/Hao Wang
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9:06 Miniprep the 2-4O->1-18P plasmids
11:52 Digest the plasmids of 2-4O->1-18P and 1-23L:
Digestion System Insert: 20μL;
Plasmids | 5μL |
EcoR1 | 1μL |
Spe1 | 1ul |
Buffer 1*H | 2μL |
dd H2O | 11μL |
Digestion System Vector: 20μL;
Plasmids | 5μL |
EcoR1 | 1μL |
Xbal1 | 1ul |
Buffer 1*M | 2μL |
dd H2O | 11μL |
12:13 Reaction Start
20:08 Prepare to Ligation
20:17 Ligation start