IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/08/Min Lin

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The Primer(Made of Standard Prefix and Suffix arrived).
Add ddH2O to each tube.

PCR tetR plasmid (MasterMix) to see whether the primers work.
PCR protocol.
PKU 20090708 Min Lin 1.JPG
( the Band is too narrow which means that the PCR is not done very well)

Try Colony PCR
No Signal

Go to Lingli’s Lab, do gradient PCR.
52, 53, 54, 55, 56, 57, 62, 64

All of the temps works well.
PKU 20090708 Min Lin 2.JPG

It turns out that the thermocycler in our lab has no hot cap. So the liquid is on the tube cap when exposed to 94 centigrade.