IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/07/Hao Wang

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0:34 The digestion reaction begins

7:46 add 0.4uL cip and 2ul buffer to the vector system

8:07 the result come out as follows:
PKU 20090707 Hao Wang 1.JPG

18:03 Recycle the fragments.

19:40 Ligate the 2-4O insert to 1-18P vector:

Insert 3μL
Vector 1μL
Buffer 10* 1μL
Ligase 1uL
dd H2O 4μL

19:48 Ligation start

23:44 Begin the transformation of the ligation plasmid