IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/06/Min Lin

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MiniPrep tetR standard parts plasmid.(BBa_C0040)
Get 6 standard rbs plasmids From ShenShan.

21:40
Digest rbs

SpeI 1.5ul
PstI 1.5ul
10xH buffer 5ul
Plasmid 5ul
ddH2O 37ul

Digest tetR

XbaI 1ul
PstI 1ul
10xM buffer 2ul
Plasmid 5ul
ddH2O 11ul