IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/06/13/Hao Wang

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with LinMin

15:00 make the culture medium, repair
computer, search Parts:

Parts Code Location Location Plasmid Containment Description
BBa_E0240 Kit Plate 1 12M Psb1A2 GFP rbs-repoter Medium rbs
BBa_E0840 Kit Plate 1 12O Psb1A2 GFP rbs-repoter Strong rbs
BBa_R0080 Kit Plate 1 12E Psb1A2 Ara Promoter
BBa_C0062 Kit Plate 1 4O Psb1A2 Lux R gene No LVA
BBa_C0051 Kit Plate 1 4E Psb1A2 C1 repressor With LVA
BBa_C0179 Kit Plate 2 8M Psb1A2 lasR activator No LVA
BBa_C0079 Kit Plate 1 14J Psb1A2 lasR activator With LVA
BBa_R0079 Kit Plate 1 12A Psb1A2 LasR/Pal promoter

16:55 dissolve the plasmids, prepare to add 15uL ddH2O
transform
21:20 Transform plasmids above