Electrophoresis and Gel Extraction for T7ptag+1-23L insert
Result (from left to right)
T7ptag①, T7ptag②, Marker
Gel: T7ptag+1-23L 0.125gPN volume: 375μL
Ligate the T7ptag+1-23L insert and 9 RBS vector
System
3μL |
insert
|
1μL |
RBS vector
|
1μL |
10× Ligase buffer
|
1μL |
Ligase
|
4μL |
ddH2O
|
10μL |
Total
|