IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/09/Min Lin
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10:00
Help ZhangHaoQian, ZhangGuoSheng, Wushuke and WangHao do assessment of the colonies(Colony PCR). Make mixture for Colony PCR.
There are 96 tubes in the PCR machine.
12:00
PCR T7ptag to standarlize the T7ptag gene.( the primer sequence here)
system here.
Condition here.
Use the machine in the New Life Science Building, ZebraFish Lab.
Do Gradient PCR, from 54 to 65 centigrade.
Use PFU
54,55,56,57,58,59,60,61,62,63,64,65.
Recycle by GEL.
All of the temps worked, and 65 is the best temp.
