IGEM:PKU Beijing/2009/Notebook/AND Gate 2/2009/07/23/Zhenglin Yang

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10:40 PCR production purify

12:30 Double digestion for purified T3 Promoter PCR Production

16:30 Purify the digestion production

17:30 Ligation of T3 promoter and GFP vector

18:00 Miniprep for T3 Polymerase

21:30 Ligation of T3 promoter and TetR backbone (T3 promoter parts)

22:00 Transformation of T3 promoter + GFP

23:00 PCR for T7 polymerase

23:30 Plate cultivation of T3 promoter + GFP