IGEM:PKU Beijing/2009/Notebook/AND Gate 2/2009/07/21/Zhenglin Yang
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1:00 Double digestion for purified T3 Polymerase PCR Production
8:00 Purify the digestion production
9:00 Ligation of T3 polymerase and TetR backbone
14:30 Transformation
16:15 Plate cultivation
