IGEM:PKU Beijing/2009/Notebook/AND Gate 2/2009/07/19/Zhenglin Yang
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Received the Three Counter Plasmid
13:00 Transformation:
Transform the three counters plasmid into the DH5α cell and apply the bacteria template to K+ plate
15:00 Plate cultivation
37℃ plate culture
16:00 Double digestion for GFP vector
23:00 Tube cultivation
Pick single clones on the plate and 37℃ tube culture in shaker