IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/14/Zhangs

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Ligate the 1-5J RBS+CI+1-23L insert and T7 promoter vector
System

3μL insert
1μL T7p vector
1μL 10× Ligase buffer
1μL Ligase
4μL ddH2O
10μL Total