IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/14/Min Lin

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MiniPrep low copy plasmid of the bistable plasmid.
Concentration = 130ng/ul
Enzyme Digestion with

EcoRI 1ul
PstI 1ul
10xH buffer 2ul
Plasmid 7ul
ddH2O 9ul


Low copy bistable plasmid is lost.
Ask Louchunbo about it, the low copy plasmid is not standarlized.