IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/14/Min Lin

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16:00
MiniPrep low copy plasmid of the bistable plasmid.
Concentration = 130ng/ul
Enzyme Digestion with

EcoRI 1ul
PstI 1ul
10xH buffer 2ul
Plasmid 7ul
ddH2O 9ul

GEL:

Low copy bistable plasmid is lost.
Ask Louchunbo about it, the low copy plasmid is not standarlized.