IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/12/Zhangs
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Determine the concentration of 1-5J plasmid by spectrophotometer.
1-5J② 7.55ng/μL×50=377.5ng/μL
1-5J③ 2.53ng/μL×50=126.5ng/μL
Double digestion for 1-5J+CI+1-23L insert again
1.5μL | XbaI |
1.5μL | PstI |
2μL | 10×M buffer |
3μL/7μL | 1-5J② plasmid/1-5J③ plasmid |
12μL/8μL | ddH2O |
20μL Total |