IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/12/Min Lin

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GEL to see the PCR product
PKU 20090712 Min Lin 1.JPG
There are contaminations.
Another PCR to see whether it is caused by the contamination of the ddH2O
PKU 20090712 Min Lin 2.JPG
There are still contaminations. Do not know the reason.

Wushuke’s gel also has the contamination bands, even in those negative control without template.
It is supposed to be caused by primer contamination.

MiniPrep T7ptag(standardized) plasmid, T7ptag+terminator plasmid, and SupD(Standarlized) plasmid.
Send for sequencing