IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/07/Min Lin

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GEL to assess the digestion
PKU 20090707 Min Lin 1.JPG
CIP the 6 rbs vectors.
Add to the digestion product 5ul CIP Buffer and 1ul CIAP

Run a GEL to recycle the insert of tetR.
The Hole is too large, the band is missing. (Never use the largest Cone for 20ul recycle any more)

Digest the tetR plasmid again.