IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/06/Hao Wang

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19:51 Miniprep the plasmids:

2-4O LuxR+RBS Amp+
1-18P P(cat) Kan+

21:08 Digest the plasmids of 2-4O and 1-18P:
Digestion System Insert: 20μL;

Plasmids 5μL
Pst1 1μL
Xbal1 1ul
Buffer 1*M 2μL
dd H2O 11μL

Digestion System Vector: 20μL;

Plasmids 5μL
Pst1 1μL
Spe1 1ul
Buffer 1*H 2μL
dd H2O 11μL