IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/06/Hao Wang
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19:51 Miniprep the plasmids:
| 2-4O | LuxR+RBS | Amp+ |
| 1-18P | P(cat) | Kan+ |
21:08 Digest the plasmids of 2-4O and 1-18P:
Digestion System Insert: 20μL;
| Plasmids | 5μL |
| Pst1 | 1μL |
| Xbal1 | 1ul |
| Buffer 1*M | 2μL |
| dd H2O | 11μL |
Digestion System Vector: 20μL;
| Plasmids | 5μL |
| Pst1 | 1μL |
| Spe1 | 1ul |
| Buffer 1*H | 2μL |
| dd H2O | 11μL |
