IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/28/Shan Shen

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14:00 Miniprep the plasmids of T7 promoter, 1-12M, 1-12O.
The concentration of the plasmids:

T7 promoter 305ng/μL
1-12M 280ng/μL
1-12O 295ng/μL

15:00 Digest the plasmids of T7 promoter, 1-12M and 1-12O
Digestion System for plasmids of T7 promoter: 20μL

Plasmids 3μL
Pst1 1μL
Buffer 1*H 2μL
dd H2O 14μL

Digestion System for plasmids of 1-12M and 1-12O: 20μL

Plasmids 4μL
Xba1 1μL
Pst1 1μL
Buffer 1*M 2μL
dd H2O 12μL

17:10 Start to digest.
22:00 Double digestion of plasmid of T7 promoter.
Digestion System for plasmids of T7 promoter: 20μL

Plasmids 3μL
Xba1 1.5μL
Pst1 1.5μL
Buffer 1*M 2μL
dd H2O 12μL

23:25 Electrophoresis to test the digestion efficiency (17:10).
The order and the amount of the samples: marker 10μL, plasmids of T7 promoter 10μL, digestion products of T7 promoter 10μL, plasmids of 1-12M 10μL; digestion products of 1-12M, plasmids of 1-12O, digestion products of 1-12O.
Results:
PKU 20090628 Shan Shen 1.JPG
The plasmids have been cut downright and are suitable for recycling.