IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/23/Zhangs
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Double digestion for Terminator vector
Digestion system:
| 30μL | Terminator plasmid |
| 5μL | 10×M beffer |
| 1μL | EcoRI |
| 1μL | XbaI |
| 13μL | ddH2O |
| 50μL | Total |
After 90min digestion, add: 1~2μL CIAP+5μL CIAP buffer
Ligate the CI insert and Terminator vector.
Transforming the CI insert + Terminator vector ligation product.
