IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/22/Zhangs
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Double digestion for CI insert
Digestion system:
30μL | CI plasmid |
5μL | 10×H beffer |
1μL | EcoRI |
1μL | SpeI |
13μL | ddH2O |
50μL | Total |
Electrophoresis:
50μL system + 10μL buffer
5μL marker + 1μL buffer
Sample loading order: DL2000 marker, CI1, CI2
Extraction the nucleic acids from gel.