IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/18/Min Lin
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The T7 promoter and 1-12O plate is filled with colonies, it is contaminated with uncutted vectors.
Pick 3 colonies and shake in 37 centigrade incubator.
5 Colonies grow on The Double antibiotic plate
Pick and shake in double antibiotic LB.