IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/06/13/Hao Wang
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with LinMin
15:00 make the culture medium, repair
computer, search Parts:
| Parts Code | Location | Location | Plasmid | Containment | Description |
| BBa_E0240 | Kit Plate 1 | 12M | Psb1A2 | GFP rbs-repoter | Medium rbs |
| BBa_E0840 | Kit Plate 1 | 12O | Psb1A2 | GFP rbs-repoter | Strong rbs |
| BBa_R0080 | Kit Plate 1 | 12E | Psb1A2 | Ara Promoter | |
| BBa_C0062 | Kit Plate 1 | 4O | Psb1A2 | Lux R gene | No LVA |
| BBa_C0051 | Kit Plate 1 | 4E | Psb1A2 | C1 repressor | With LVA |
| BBa_C0179 | Kit Plate 2 | 8M | Psb1A2 | lasR activator | No LVA |
| BBa_C0079 | Kit Plate 1 | 14J | Psb1A2 | lasR activator | With LVA |
| BBa_R0079 | Kit Plate 1 | 12A | Psb1A2 | LasR/Pal promoter |
16:55 dissolve the plasmids, prepare to add 15uL ddH2O
transform
21:20 Transform plasmids above
