IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/10/03

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Date: 10/3/14 People in lab: Kelsey Crossen

Title: Broth cultures of CT1-6 10/2 Start Time: 2 pm Purpose: Miniprep preparation Protocol: LTM Ed. 2 Innoculation Exceptions: Products:

Sample Label Description Source Label Quantity
BC1 10/3 hmp psB1C3 CT2 10/2 1
BC2 10/3 hmp psB1C3 CT1 10/2 1
BC3 10/3 hmp psB1C3 CT1 10/2 1
BC4 10/3 hmp with pro+RBS CT1 10/3 1
BC5 10/3 hmp with pro+RBS CT3 10/2 1
BC6 10/3 hmp with pro+RBS CT3 10/2 1
BC7 10/3 hmp with pro+RBS CT4 10/2 1
BC8 10/3 hmp with pro+RBS CT4 10/2 1
BC9 10/3 hmp with pro+RBS CT5 10/2 1
BC10 10/3 hmp with pro+RBS CT5 10/2 1
BC11 10/3 hmp with pro+RBS CT6 10/2 1
BC12 10/3 hmp with pro+RBS CT6 10/2 1

Results: Notes: Stop Time: 2:45 pm Next: Glycerol stocks of BC1-3 and miniprep of the rest

Date: 10/3/14 People in lab: Kira Buckowing

Title: Chemical Transformation of L1 and L2 10/2 Start Time: 8:30 pm Purpose: To test if the digestion and ligation of norV was successful Protocol: LTM Ed. 2 Chemical Transformation Exceptions: Products:

Sample Label Description Source Label Quantity
CT1 10/3 20 uL L1 10/2 1
CT2 10/3 200 uL L1 10/2 1
CT3 10/3 20 uL L2 10/2 1
CT4 10/3 200 uL L2 10/2 1


Results: Notes: Wrong plates used by mistake at the last step and the procedure was started over and redone (original start time ~5 pm) Stop Time: 11:30 pm Next: Innoculation of any colonies that grow