IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/10/02

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Date: 10/2/14 People in lab: Kelsey Crossen

Title: Chemical Transformation Start Time: 4:35 pm Purpose: Transform successful hmp plasmid to make glycerol stocks and transform ligated product of hmp and pro+RBS to see if it works Protocol: LTM Ed. 2 Chemical Transformations Exceptions: Products:

Sample Label Description Source Label Quantity
CT1 10/2 20 uL MP1 9/29 1
CT2 10/2 200 uL MP1 9/29 1
CT3 10/2 20 uL L3 9/26 1
CT4 10/2 200 uL L3 9/26 1
CT5 10/2 20 uL L3 9/26 1
CT6 10/2 200 uL L3 9/26 1

Results: Notes: Stop Time: 8:23 pm Next: Liquid cultures of any growth for minipreps

Date: 10/2/14 People in lab: Kira Buckowing

Title: Digest and ligatioin of norV and psB1C3 backbone; Prep of hmp plasmids for cDNA lab sequencing Start Time: 4:50 pm Purpose: To see if the hmp product is correct and to see if the sample of norV from 11/25/13 are correct and can be used at all Protocol: LTM Ed. 2 Digestion and Ligation and Vanessa's instructions for cDNA Exceptions: Products:

Sample Label Description Source Label Quantity
L1 10/2 Ligation of A1 and backbone A1 11/25/13 1
L2 10/2 Ligation of norV and backbone A2 11/25/13 1




Results: Notes: cDNA products stored in the freezer for sequencing Stop Time: 7:30 pm Next: Transformation of ligations to see if anything grows and sequencing