IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/09/29

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Date: 9/29/14 People in lab: Kira Buckowing

Title: Minipreps Start Time: 12:15 pm Purpose: To purify the wanted plasimd from liquid cultures Protocol: LTM Ed. 2 Miniprep Exceptions: Products:

Sample Label Description Source Label Quantity
MP1 9/29 Purrified hmp plasmid BC1 9/28 1
MP2 9/29 Purified hmp plasmid BC6 9/28 1




Results: Notes: Stop Time: 2:20 pm Next: Digest of plasmid and gel to see if a vector and insert part are clearly visible

Date: 9/29/14 People in lab: Kelsey Crossen

Title: Nanodrop of miniprep product Start Time: 3:15 pm Purpose: Find out concentration of miniprepped hmp plasmids Protocol: LTM Ed. 2 Nanodrop Exceptions: Products:

Sample Label Description Source Label Quantity
--- ng/uL = 42.4; 260/280 = 2.13; 260/230 = 2.11 MP1 9/29 1
--- ng/uL = 26.2; 260/280 = 2.02; 260/230 = 1.61 MP2 9/29 1




Results: Notes: Stop Time: 3:30 pm Next: Digest to confirm presence of hmp

Date: 9/29/14 People in lab: Kelsey Crossen

Title: Digest miniprep plasmids to confirm identity Start Time: 3:30 pm Purpose: To confirm the presence of hmp in the ligated plasmid Protocol: LTM Ed. 2 Digest and Gels Exceptions: Products:

Well 1 2 3 4 5 6 7 8
- Ladder - D1 - D2

Products: Results: Two bands visible for D1 ~1100 bp and ~2200 bp, correspondign to hmp and psB1C3 bacbone Notes: Stop Time: 5:50 pm Next: Re-transform miniprep product into comp cells to make glycerol stocks