IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/09/26

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 9/26/14 People in lab: Kelsey Crossen Title: Ligation of hmp into psB1C3 and pro+RBS backbones Start Time: 2:15 pm Purpose: To have successful ligated products Protocol: LTM Ed. 2 Ligation Exceptions: L2 and L3 have 4 uL of vector and insert Products: Sample Label Description Source Label Quantity L1 9/26 hmp in psB1C3 D1 and D4 9/25 1 L2 9/26 hmp in psB1C3 D1 and D4 9/25 1 L3 9/26 hmp in pro+RBS D2 and D3 9/25 1 L4 9/26 hmp in pro+RBS D2 and D3 9/25 1 Results: Notes: Stop Time: 2:50 pm Next: Chemical Transformations