IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/09/25

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Date: 9/25/14 People in lab: Kelsey Crossen

Title: Digestions of hmp and vectors Start Time: 2 pm Purpose: Digest hmp from TOPO vector, chlor bb with pro+RBS and iGEM plasmid (psB1C3) Protocol: LTM Ed. 2 Digestion Exceptions: psB1C3 digestion done at half value due to low concentration Products:

Sample Label Description Source Label Quantity
D1 9/25 hmp digested with E and P MP4 7/30 1
D2 9/25 hmp digested with E and P MP4 7/30 1
D3 9/25 pro+RBS bacbone digested with S and P MP2 8/9 1
D4 9/25 shipping backbone digested with E and P 2013 psB1C3 1

Results: Notes: D1 needs to be ligated into D4 and D2 needs to be ligated into D3 Stop Time: 4:25 pm Next: