IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/09/01

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 9/1/14 People in lab: Kira Buckowing, Joshua Heath Title: nosZ PCR and making competent cells Start Time: 12:30 pm Purpose: To have chemically competent cells so the project can progress and to test the odd results from the previous PCR's of nosZ Protocol: LTM Ed. 2 Exceptions: 2uL DNA and 2.5 uL primers used Products: Sample Label Description Source Label Quantity PCR1 9/1 Genomic prep GP1 8/16 1 PCR2 9/1 Resuspended PAO1 Cells PAO1 Temp 8/16 1 Results: Notes: Stop Time: 8:15 pm Next: Gel extraction of PCR products and testing of comp cells