IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/27
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Cyanobacteria Mediated Filtration of Coal Flue Gas | Main project page Previous entry Next entry | ||||||||||||||||||||
Date: 8/27/14 People in lab: Kelsey CrossenTitle: Transformation of hmp and pro+RBS backbone and RFP in chlor resistant backbone to test comp cells Start Time: 2 pm Purpose: To test if comp cells are working by running a control with the target reaction for the project Protocol: LTM Ed. 2 Chemical Transformation Exceptions: 1.5 uL RFP and 1.5 uL Ligation product used and run in the same transformation Products:
Results: (8/29) No growth - comp cells are deemed bad Notes: DH5 alpha cells used from bottom shelf of -80 C freezer Stop Time: 5 pm Next: Comp cells need to be remade
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