IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/19

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Date: 8/19/14 People in lab: Emily Puleo, Kelsey Crossen

Title: Chemical Transformation Start Time: 3:45 pm Purpose: To transform ligated product into comp cells Protocol: LTM Ed. 2 Chemical Transformation Exceptions: Products:

Sample Label Description Source Label Quantity
CT1 8/19 20 uL L1 8/18 1
CT2 8/19 200 uL L1 8/18 1
CT3 8/19 20 uL L1 8/18 1
CT4 8/19 200 uL L1 8/8 1

Results: (8/20) no growth present, only misleading air bubbles that looked like colonies; retry with differences in vector/insert ratio or new buffer Notes: Stop Time: 6:14 pm Next: Start liquid cultures from colonies that grow