IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/16

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Date: 8/16/14 People in lab: Kira Buckowing

Title: Genomic Prep and PCR of PAO1 Start Time: 10 am Purpose: To obtain usable components needed for nosZ Protocol: LTM Ed. 2 PCR; DNA, RNA and protein purification kit manual Exceptions: Products:

Sample Label Description Source Label Quantity
GP1 8/16 Genomic prep of PAO1 cells reconstituted from a dried up plate --- 1
PAO1 Temp 8/16 PCR of resuspended PAO1 cells to check for usability --- 1

Results: Notes: Incubation for Genomic Prep ~ 3hrs, optional RNA clean up step followed Stop Time: 3 pm Next: Gel electrophoresis of PCR to test, PCR of GP1 to check viability