IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/11

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Date: 8/11/14 People in lab: Emily Puleo, Joshua Heath

Title: Gel Extraction of hmp Start Time: 1 pm Purpose: Isolate hmp from vector Protocol: LTM Ed. 2 Gel Extraction Exceptions: Products:

Sample Label Description Source Label Quantity
8/11 GE1 hmp 8/6 D1 1
8/11 GE2 hmp 8/6 D2 1




Results: Notes: Stop Time: 1:45 pm Next: Ligate with pro+rbs part

Date: 8/11/14 People in lab: Emily Puleo, Joshua Heath

Title: Digest pro+RBS and TT Start Time: 1:45 pm Purpose: Prep for ligation Protocol: LTM Ed. 2 Digest Exceptions: For pro+RBS = 15.5 uL DNA, 5 uL milliQ, 1 uL speI and pstI; for TT = 1 uL xbal and pstI Products:

Sample Label Description Source Label Quantity
8/11 D1 pro+RBS digested with S and P 8/9 MP1 1
8/11 D2 pro+RBS digested with S and P 8/9 MP2 1
8/11 D3 TT digested with X and P 8/9 MP3 1
8/11 D4 TT digested with X and P 8/9 MP4 1


Results: Notes: Stop Time: 3:45 pm Next: Nanodrop

Date: 8/11/14 People in lab: Emily Puleo, Joshua Heath

Title: Nanodrop Start Time: 3:55 pm Purpose: Measure the purity and concentration of the samples Protocol: LTM Ed. 2 Nanodrop Exceptions: Products:

Sample Label Description Source Label Quantity
--- ng/uL = 17.5; 260/280 = 11.16; 260/230 = 0.11 8/11 GE1 1
--- ng/uL = 30.9; 260/280 = 9.79; 260/280 = 0.6 8/11 GE2 1




Results: Notes: Stop Time: 4:05 pm Next: Ligation