IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/30

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Date: 7/30/14 People in lab: Joshua Heath

Title: Miniprep and Nanodrop Start Time: 10:40 am Purpose: Purify DNA and check quality Protocol: LTM Ed. 2 Miniprep and Nanodrop Exceptions: Products:

Sample Label Description Source Label Quantity
7/30 MP 1 Water 7/29 BC1 1
7/30 MP 2 Water 7/29 BC2 1
7/30 MP 3 Elution 7/29 BC3 1
7/30 MP 4 Elution 7/29 BC4 1


Results: MP 1 - ng/uL = 171.1, 260/280 = 1.92, 260/230 = 3.02; MP 2 ng/uL = 171.6, 260/280 = 1.9, 260/230 = 3.17; MP 3 ng/uL = 161.2, 260/280 = 1.92, 260/230 = 2.21; MP 4 ng/uL = 186.1, 260/280 = 1.9, 260/230 = 2.15 Notes: Stop Time: 1:40 pm Next: Digest with EcoRI

Date: 7/30/14 People in lab: Joshua Heath

Title: Digestion Start Time: 2 pm Purpose: To separate hmp Protocol: LTM Ed. 2 Digestion Exceptions: Products:

Sample Label Description Source Label Quantity
7/30 D1 hmp digested with EcoRI 7/30 MP1 1
7/30 D2 hmp digest with EcoRI 7/30 MP2 1
7/30 D3 hmp digested with EcoRI 7/30 MP3 1
7/30 D4 hmp digested with EcoRI 7/30 MP4 1


Results: Notes: Stop Time: 4:15 pm Next: Gel

Date: 7/30/14 People in lab: Joshua Heath

Title: Gel Electrophoresis Start Time: 5:50 pm Purpose: To identify the hmp fragment Protocol: LTM Ed. 2 Gels Exceptions: Products:

Well 1 2 3 4 5 6 7 8 9 10
- Ladder - D1 - D2 - D3 - D4 -

Products: Results: Notes: Stop Time: 8:10 pm Next: