IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/02/25

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 02/25/14 People in lab: Levi Palmer + helpers Title: PCR of hmp, gel electrophoresis, TOPO TA cloning Start Time: 12:00PM Purpose: To amplify hmp gene and put into a vector for further amplification Protocol: LTM ed. 2, Invitrogen TOPO-TA cloning manual Products: Sample Label Description Source Label Quantity hmp A 2/25 PCR of hmp with suffix&prefix E. coli 8/6/13 1 2/25 LP ET hmp TOPO hmp in TOPO-TA vector on xgal hmp A 2/25 1 Well 1 2 3 4 5 6 7 8 Sample Ladder hmp Results: hmp visible on gel Notes: Transformation arced b/c did not dilute the transformation before electroporating(TOPO has high salt content) Stop Time: 6:00 PM Next: Blue/white colony screening