IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/01/30

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 01/30/14 People in lab: Levi Palmer Title: PCR of norCB adding prefix/suffix then electrophoresis Start Time: 10:15 AM Purpose: To PCR amplify norCB and add prefix and suffix and confirm on a gel Protocol: LTM ed. 2 Exceptions:1. 7.5 uL of p. aeru suspension, 2. Final primer conc. <.1uM Products: Sample Label Description Source Label Quantity norCB 1 norCB gene with prefix and suffix P aeru 7/31 2 Well 1 2 3 4 5 6 7 8 Sample Ladder Ladder norCB1 norCB2 Results: No product visible Stop Time: 4:00 PM Next: Retry with different temperatures