IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/30

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 09/30/13 People in lab: Levi Palmer Title: Transform backbone into competent cells Start Time: 4:12 PM Purpose: To test for competence of cells and amplify amp backbone Protocol: LTM ed. 2 Products: Sample Label Description Source Label Quantity 9/30 Amp Backbone CT E. coli transformed with Amp backbone L LP 9/28 2 9/30 pUC19 BB CT E. coli transformed with pUC19 control plasmid pUC 19 DNA 2 Results: 10/01: Transformations did not work. Notes: Performed with both amp and pUC 19 plasmids Stop Time: 7:00 PM Next: Check for success, if succesful, inoculate amp cells into LB broth.