IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/14

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Date: 09/14/13 People in lab: Hannah Frye

Title: PCR of norCB an nosZ for TOPO TA cloning

Start Time:

Purpose: Add iGEM prefix&suffix to the genes, then TOPO-TA clone to amplify

Protocol: 1. Resuspended P. aeru cells in0.5mL milliQ 2. Spun down Taq Master Mix 3. Mixed - 12.5 uL Taq MM, 7.5 uL P. Aeru, 2.5 uL F primer, 2.5 uL R primer 4. Spun down mixture 5. Annealing Temp: 65C, Elongation Time: 7 min

Products:

Sample Label Description
norCB 9/14 norCB with prefix/suffix
nosZ 9/14 nosZ with prefix/suffix

Stop Time:

Next: Gel electrophoresis