IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/07

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 08/07/13 People in lab: Blythe Ferriere Title: Gel Electrophoresis of 8/6/13 A1-A6 PCR reactions Start Time: 8:45 AM Purpose: To check for norV gene product with prefix&suffix from PCR amplification. Protocol: LTM ed. 2 pg 45-47 Well 1 2 3 4 5 6 7 8 Sample Ladder 8/6/13 A1 8/6/13 A2 8/6/13 A3 8/6/13 A4 8/6/13 A5 8/6/13 A6 Ladder Products: N/A Results: PICTURE Notes: Stop Time: 12:33 PM Next: TOPO cloning of 8/6/13 PCR products Date: 08/07/13 People in lab: Blythe Ferriere Title: TOPO cloning of PCR product and chemical transformation Start Time: 2:45 PM Purpose: To TOPO clone 8/6/13 A1 norV PCR product and chemically transform to amplify gene product Protocol: TOPO cloning: Fresh PCR product 4uL, PCR TOPO vector 1uL. 1. Mix gently and incubate for 5 minutes at room temperature. Transformation: LTM ed. 2 pg. 42 Exceptions: 1. 2uL of TOPO cloning reaction product 2. 1uL pUC19 control standard 3. Plate on amp+Xgal Products: Sample Label Description Source Label Quantity Notes: PCR product was a day old. 1 hour 35 min recovery time. Stop Time: 3:40 PM Next: TOPO cloning of 8/6/13 PCR products