IGEM:JohnsHopkins/2008/Budget

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Item Supplier Amount [ ] if app Quantity Cat. # Cost
Restriction Enzymes $1,500
DNA synthesis $4,000
Subtotal 5,500
PCR supplies
Taq pol NEB 4000 units 5000 units/ml 1 M0273X 400
Standard Taq Reaction Buffer NEB 6mL 10X 4 B9014S 40
96-Well Nonskirted PCR Plates Fisherbrand 25 plates 2 08-408-220 143.58
8-well; 0.2mL; Color: Natural Fisherbrand 125 tubes 2 08-408-237 316.62
Adenosine 5′-triphosphate disodium salt Grade II, ≥99%, crystalline, from microbial source Sigma 5g 1 A3377 55.4
Guanosine 5′-triphosphate sodium salt hydrate ≥95% (HPLC), powder Sigma 100mg 1 G8877 95
Cytidine 5\'-triphosphate disodium salt hydrate BioChemika, ≥90.0% (HPLC) Sigma 100mg 1 30320 62.7
Thymidine 5′-triphosphate sodium salt ~97% Sigma 50mg 1 T0251 118
Subtotal 1,231
Culturing Supplies
Tryptone Peptone(Bacto-Tryptone) Difco 2kg 1 211699 263.9
Yeast Extract Difco 2kg 1 212720 326.9
NaCl Sigma 1kg 1 S6191 53.8
Dextrose (D-Glucose) J.T Baker 12 KG 1 1920-07 457.82
Potassium Chloride Sigma 1 kg 1 P9541 49.5
Magnesium Sulfate (anhydrous) Sigma 100g 1 246972 23.4
Peptone, Bacto Difco 2kg 1 211820 286.7
Bacto-Agar Difco 2kg 1 214030 658.05
YNB w/o Amino Acids Difco 100g 1 291940 79.6
96-Well Plates Sterile Fisherbrand 40 plates 1 21-377-205 208
Raised Ridge; 100 O.D. x 15mmH Petri Dishes Fisherbrand 500 plates 2 08-757-12 300.1
Ampicillin anhydrous, 96.0-100.5% (anhydrous basis) Sigma 25g 1 A9393 157
G 418 disulfate salt powder, cell culture tested Sigma 1g 1 A1720 88.9
Subtotal 2953.67
Gel Electrophoresis supplies
EDTA J.T Baker 500g 1 8994-01 212.5
2-Log DNA Ladder NEB 500 μg 1,000 μg/ml 1 N3200L 244
Xylene Cyanol Sigma 10g 1 X4126 40.8
Bromophenol Blue Sigma 5g 1 B8026 24.3
Glycerol Sigma 500mL 1 G5516 31.2
Ethidium Bromide Sigma 5g 1 E7637 105
Taurine Sigma 1kg 1 T0625 403
Agarose, Standard, Low Electroendosmosis (EEO), ULTRAPURE BIOREAGENT J.T Baker 500g 1 A426-07 651.5
Tris -Base J.T Baker 5kg 1 4109-06 363.85
Subtotal 2076.15
Grand total 11,761



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