IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/29

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  • Repeated the Digestion of E0420. It worked fine.
  • Did the Ligation of the composite parts as follows:
  • J23119 + S03879 ----> 001
  • K145151 + B0014 ----> 018
  • J23119 + E0420 -----> 019
  • R0010 + E0420 ------> 502
  • K117005 + K145151 --> 503
  • Transformed all these parts in DH5a cells.