IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/13

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  • Discussion with Dr. Madhulika regarding the PCR approach.
  • Primers designed
  • transformations of B0034 and pSB3C5 did not work; the other 4 worked fine
  • Transformed pSB3C5 again in DH5a.
  • Inoculated the 4 plasmids in 2xyt medium for the miniprep.