IGEM:Imperial/2010/2010/07/13

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Wiki tutorial

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Table of ideas

Table of ideas
Stimulus Signalling Effector 1 (Protease) Effector 2 (Dye,Enz) Pigment biosynthetic pathways
Parasites: Florian & Harrriet 2 components systems transcr sigma 54 2 colourless Billins
Fv-fragment detection system logic gates Activation (phosphorylation) Enz-in-pathway Anthocyanins
  • short pathways
  • ensure - colourless -> colour
Vibrio cholera Target proteases to use: Piotr, Nick, Kyasha
  • not many AA
  • non-toxic
  • can work in E.Coli
  • quantize speed, efficiency
Protein scaffold: Maddie
Toluene, benzene, xylene rings 2C DNA binding prot release

Fret pairs as back up for effectors