IGEM:IMPERIAL/2009/Feedback & Debriefs/Feedback 21 7

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Tuesday 21st July 2009 - Presentation to Profs. & Advisors

Prof:

  • Need a project overview
  • Presentation does not follow the engineering process

- need 2-3 slides focusing on this

  • PKU

- there is a real problem/real emotional message to get across
- is a novel application!
Focus on one application with the method being compatible to other applications

Advisor:

  • Need details of the enzyme

- where it works
- where the conversion occurs etc.

  • What is the recommendation per module?!

- Need a summary after each

Prof:

  • More modelling

- Have the PKU pathway

Prof:

  • How to trigger encapsulation process?
  • How feasible is the killing strategy?


Prof:

  • Which track are we aiming to enter?
  • How close is this to previous projects?
  • Need something that actually works with results

- killing strategy
- encapsulation

Prof:

  • Match the thresholds and sensitivities

- can be done
- use GFP reporter with an optical read out?

Advisor:

  • Produce 2 chassis/cells?
  • Explore module, get genes, find existing bacillus/E.coli chassis, one with a good coat formation and one that can produce the drug.


Prof:

  • Create new self encapsulation module
  • Take out the biological pathway and place in Bacillus
  • Drill down
  • Make sure it’s quality controlled before consumption

Prof:

  • Be brutal in testing the risk for each module
  • Try to order parts ASAP

- waiting time should not be a limiting factor

  • Come on a lot since Friday

- got bones of a good project
- like idea of modules

Advisor:

  • Write a high level specification page (case study almost?!)
  • Look at an E.coli apoptotic mechanism

- there is one

Prof:

  • What about the proteases?!

- Use protein deficient strain?

  • Still a lot of yes/no points we need to sort out


Deadline for BioBrick design: Friday

Prof & Advisor:
What is the critical path in the project?

Prof:

  • Amalyse, protease, lipase deficiency?
  • Enzyme deficiencies in the gut
  • Need the pros and cons of different applications

- where are they on they wiki?
- convince what you want to pick is feasible/efficient
- what are the mechanisms (existing)
- why do we need the enzyme?
- regulation, control, how does it know what/how much to make?
- give details, details, details!