IGEM:IMPERIAL/2007/Projects/In-Veso/Implementation/Results1.1

From OpenWetWare
Jump to: navigation, search

Results Summary

  • Vesicles were formed on 15th August '07, AM. Size range: 2 - 43μm.
  • We can distinguish air bubbles from vesicles as air bubbles tend to only have a thin dark line around its borders. In contrast, the vesicles that we observed were much thicker, and emitted different bands of various shades when placed under phase contrast.
  • A mistake was made when we used 2ml of emulsion instead of 2ml DOPC-mineral oil suspension followed by 100μl of emulsion. However, we still managed to capture images of vesicle formation as shown.
IC07 image077.jpg IC07 image078.jpg IC07 image079.jpg
Photos taken with a portable camera through the microscope eye-piece. Setup: 100x oil immersion objective, with phase contrast.