IGEM:IMPERIAL/2007/Experimental Design/Phase2/Results 1.2

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Result Summary

  • Tests were carried out on the following concentrations of GFP:
    • 3.7µM, 1.8µM, 1µM, 0.5µM, 0.1µM, 0.05µM, 0.025µM, 0.0125µM and 0µM
Calibration curve for GFP in vitro

The experiment was carried out to calibrate the response of GFP fluorescence to different concentrations of GFP, in-vitro. [GFP] and fluorescence would be expected to be directly proportional as they have a linear relation at certain concentrations.

The results obtained correlate with what was expected as the graph of fluorescence against [GFP] is a sraight line through the origin. The gradient of this graph is almost 1.5 times the gradient for GFP in-vivo, as seen in the characterisation of part F2620. This means that a particular concentration of GFP in-vitro has 1.5 times more fluorescence than the same concentration of GFP in-vivo.

Complete set of results can be found here: